Difference between revisions of "Texas Xenopus Genome Project/XENLA SA09023"

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(Created page with '== BAC_2kb == * Sequencing 96 BAC clones from CHORI-219, with 2kb mate-pair library construction. * XENLA:XENLA_seq.20091124.BAC_2kb_rmapper.pdf * [[:XENLA:XENLA_seq.2009112...')
 
(BAC_2kb_F3)
 
(15 intermediate revisions by 2 users not shown)
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 +
== Overall ==
 +
* [[xdata:XENLA_seq.20091211.SA09023_Qscore.pdf]]
 +
 
== BAC_2kb ==
 
== BAC_2kb ==
 
* Sequencing 96 BAC clones from CHORI-219, with 2kb mate-pair library construction.
 
* Sequencing 96 BAC clones from CHORI-219, with 2kb mate-pair library construction.
* [[:XENLA:XENLA_seq.20091124.BAC_2kb_rmapper.pdf]]
+
* [[xdata:XENLA_seq.20091124.BAC_2kb_rmapper.pdf]]
* [[:XENLA:XENLA_seq.20091124.BAC_2kb_matepair.pdf]]
+
* [[xdata:XENLA_seq.20091124.BAC_2kb_matepair.pdf]]
  
 
=== BAC_2kb_F3 ===
 
=== BAC_2kb_F3 ===
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** After 35 bp : 945,355
 
** After 35 bp : 945,355
 
** Before 35 bp : 386,928
 
** Before 35 bp : 386,928
 +
* Reads without repeats (pBACGK1.1 + RepBase 15.05) : 34,079,501 (expected coverage: 177x)
 
* '''Reads with average Qscore >= 15 : 20,607,858'''
 
* '''Reads with average Qscore >= 15 : 20,607,858'''
 
* Reads with minimum Qscore >= 15 : 379,916
 
* Reads with minimum Qscore >= 15 : 379,916
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** After 35 bp : 827,335
 
** After 35 bp : 827,335
 
** Before 35 bp : 294,577
 
** Before 35 bp : 294,577
 +
* Reads without repeats (pBACGK1.1 + RepBase 15.05) : 34,246,323 (expected coverage: 177x)
 
* '''Reads with average Qscore >= 15 : 22,050,059'''
 
* '''Reads with average Qscore >= 15 : 22,050,059'''
 
* Reads with minimum Qscore >= 15 : 1,549,458
 
* Reads with minimum Qscore >= 15 : 1,549,458
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== BAC_5kb ==
 
== BAC_5kb ==
 
* Sequencing 96 BAC clones from CHORI-219, with 5kb mate-pair library construction.
 
* Sequencing 96 BAC clones from CHORI-219, with 5kb mate-pair library construction.
* [[:XENLA:XENLA_seq.20091124.BAC_5kb_rmapper.pdf]]
+
* [[xdata:XENLA_seq.20091124.BAC_5kb_rmapper.pdf]]
* [[:XENLA:XENLA_seq.20091124.BAC_5kb_matepair.pdf]]
+
* [[xdata:XENLA_seq.20091124.BAC_5kb_matepair.pdf]]
  
 
=== BAC_5kb_F3 ===
 
=== BAC_5kb_F3 ===
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== WG_5kb ==
 
== WG_5kb ==
 
* Sequencing whole genome from ''X. laevis'' strain J (MHC inbred), with 5kb mate-pair library construction.
 
* Sequencing whole genome from ''X. laevis'' strain J (MHC inbred), with 5kb mate-pair library construction.
* [[:XENLA:XENLA_seq.20091124.WG_5kb_rmapper.pdf]]
+
* [[xdata:XENLA_seq.20091124.WG_5kb_rmapper.pdf]]
* [[:XENLA:XENLA_seq.20091124.WG_5kb_matepair.pdf]]
+
* [[xdata:XENLA_seq.20091124.WG_5kb_matepair.pdf]]
 
+
* An alternate mapping to the ''X. tropicalis'' genome: [[xdata:xlaevis to trop histogram.pdf]]
 +
** Also check [[xdata:XENLA_seq.20091203.WG_5kb_XENTR.pdf]]
 
=== WG_5kb_F3 ===
 
=== WG_5kb_F3 ===
 
* Files
 
* Files
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** SA09023_MPQuad_X_laevis_WB_R3_QV.qual (6.7 GB; 1.9 GB gzipped)
 
** SA09023_MPQuad_X_laevis_WB_R3_QV.qual (6.7 GB; 1.9 GB gzipped)
  
* Total reads: 44,888,424
+
* Total reads: 44,888,428
 
* Reads without no-call ('.' in .csfasta, '-1' in .qual) : 44,518,144
 
* Reads without no-call ('.' in .csfasta, '-1' in .qual) : 44,518,144
 
* Reads with no-call : 370,284
 
* Reads with no-call : 370,284
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* '''Reads with average Qscore >= 15 : 34,733,020'''
 
* '''Reads with average Qscore >= 15 : 34,733,020'''
 
* Reads with minimum Qscore >= 15 : 3,013,026
 
* Reads with minimum Qscore >= 15 : 3,013,026
 +
 +
----
 +
[[Category:XenopusGenome]]

Latest revision as of 13:22, 14 June 2010

Contents

Overall

BAC_2kb

BAC_2kb_F3

  • Files
    • SA09023_MPQuad_X_laevis_2kb_F3.csfasta (2.4 GB; 672 MB gzipped)
    • SA09023_MPQuad_X_laevis_2kb_F3_QV.qual (5.2 GB; 1.5 GB gzipped)
  • Total reads: 35,938,421
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 34,606,138
  • Reads with no-call : 1,333,283
    • After 35 bp : 945,355
    • Before 35 bp : 386,928
  • Reads without repeats (pBACGK1.1 + RepBase 15.05) : 34,079,501 (expected coverage: 177x)
  • Reads with average Qscore >= 15 : 20,607,858
  • Reads with minimum Qscore >= 15 : 379,916

BAC_2kb_R3

  • Files
    • SA09023_MPQuad_X_laevis_2kb_R3.csfasta (2.4 GB; 689 MB gzipped)
    • SA09023_MPQuad_X_laevis_2kb_R3_QV.qual (5.2 GB; 1.5 GB gzipped)
  • Total reads: 36,077,234
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 34,955,322
  • Reads with no-call : 1,121,912
    • After 35 bp : 827,335
    • Before 35 bp : 294,577
  • Reads without repeats (pBACGK1.1 + RepBase 15.05) : 34,246,323 (expected coverage: 177x)
  • Reads with average Qscore >= 15 : 22,050,059
  • Reads with minimum Qscore >= 15 : 1,549,458

BAC_5kb

BAC_5kb_F3

  • Files
    • SA09023_MPQuad_X_laevis_5kb_F3.csfasta (1.8 GB; 449 MB gzipped)
    • SA09023_MPQuad_X_laevis_5kb_F3_QV.qual (4.0 GB; 1.2 GB gzipped)
  • Total reads: 27,718,394
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 27,326,849
  • Reads with no-call : 391,545
    • After 35 bp : 20,043
    • Before 35 bp : 371,502
  • Reads with average Qscore >= 15 : 14,285,096
  • Reads with minimum Qscore >= 15 : 142,514

BAC_5kb_R3

  • Files
    • SA09023_MPQuad_X_laevis_5kb_R3.csfasta (1.8 GB; 487 MB gzipped)
    • SA09023_MPQuad_X_laevis_5kb_R3_QV.qual (3.9 GB; 1.2 GB gzipped)
  • Total reads: 27,719,040
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 27,465,570
  • Reads with no-call : 253,470
    • After 35 bp : 14,338
    • Before 35 bp : 239,132
  • Reads with average Qscore >= 15 : 12,236,521
  • Reads with minimum Qscore >= 15 : 493,814

WG_5kb

WG_5kb_F3

  • Files
    • SA09023_MPQuad_X_laevis_WB_F3.csfasta (2.9 GB; 856 MB gzipped)
    • SA09023_MPQuad_X_laevis_WB_F3_QV.qual (6.6 GB; 1.9 GB gzipped)
  • Total reads: 44,817,834
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 44,289,548
  • Reads with no-call : 528,286
    • After 35 bp : 30,381
    • Before 35 bp : 497,905
  • Reads with average Qscore >= 15 : 31,955,422
  • Reads with minimum Qscore >= 15 : 1,226,050

WG_5kb_R3

  • Files
    • SA09023_MPQuad_X_laevis_WB_R3.csfasta (2.9 GB; 859 MB gzipped)
    • SA09023_MPQuad_X_laevis_WB_R3_QV.qual (6.7 GB; 1.9 GB gzipped)
  • Total reads: 44,888,428
  • Reads without no-call ('.' in .csfasta, '-1' in .qual) : 44,518,144
  • Reads with no-call : 370,284
    • After 35 bp : 4,962
    • Before 35 bp : 365,322
  • Reads with average Qscore >= 15 : 34,733,020
  • Reads with minimum Qscore >= 15 : 3,013,026