Difference between revisions of "Xenopus reference"
Revision as of 10:43, 12 October 2011
All of these files are derived from XenBase (downloaded on May, 01, 2011).
Version 2. Non-redundant cDNA
- Cluster ref.v1 sequences with usearch(version 4.2.66) with %identity>80%, to remove redundancy. As a result, we got 8,164 non-redundant cDNA sequences. (XENLA_cDNA_ref.v1.uc080_fasta)
- From xlaevisMRNA.fasta file, collect all sequences that were not included in ref.v1. Mainly they are (1) annotated as RefSeq, but there is no designated gene name, or (2) not annotated as RefSeq (GenBank submitted sequences).
- Use non-redundant ref.v1 sequences (step 1) as database, and run 'usearch' with DB search + clustering mode. So non-RefSeq sequences in step 2 would be (1) clustered with ref.v1 sequence if their %id is greater than 80%, or (2) clustered as independent cluster if there is no sequences available in ref.v1 with %id>80%.
XENLA_cDNA_ref.v2.fasta (9,943 sequences)
Version 1. RefSeq of cDNA & protein
- Read gene name for each NCBI id from 'Ncbi...' file. Filter out genes with 'unnamed' in gene name field.
- Read all sequences from '.fasta' file. Convert all sequence character to upper case.
- If I find a sequence with '>gi|<gi number>|ref|<genbank accession>' header (means it is RefSeq entity), write it down.
XENLA_cDNA_ref.v1.fasta (8,879 sequences)
- Used XenBase files: NcbiMrnaXenbaseGene_laevis.txt, xlaevisMRNA.fasta
XENLA_prot_ref.v1.fasta (8,878 sequences; 'taf5' is not annotated as RefSeq in protein, although its corresponding mRNA sequence is annotated as RefSeq.)
- Used XenBase files: NcbiProteinXenbaseGene_laevis.txt, xlaevisProtein.fasta