ChungKwon2013 RFX2

Supplementary Website for Chung M-I, Kwon T, Tu F, Brooks ER, Gupta R, Meyer M, Baker JC, Marcotte EM, Wallingford JB, Coordinated genomic control of ciliogenesis and cell movement by RFX2 (submitted).

Raw data

• http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50593
• RNA-seq, Control #1 (2x100 bp, paired-end)
• RNA-seq, Control #2 (2x100 bp, paired-end)
• RNA-seq, RFX2 Knock-down #1 (2x100 bp, paired-end)
• RNA-seq, RFX2 Knock-down #2 (2x100 bp, paired-end)
• ChIP-seq, RFX2-GFP (1x50 bp, single-end)
• ChIP-seq, Control GFP (1x50 bp, single-end)

Genomic resource

• ftp://ftp.xenbase.org/pub/Genomics/JGI/Xenla6.0/ (X. laevis JGI 6.0 scaffolds)
• http://daudin.icmb.utexas.edu/pub/annot/UTA/XENLA_2012oct_cdna_longest.fa.gz (longest cDNA, 'Oktoberfest' version)
• http://daudin.icmb.utexas.edu/pub/annot/UTA/XENLA_2012oct_cdna_longest.gene.gff3.gz ('Oktoberfest' GFF file for JGI 6.0 scaffods)

Processed data

• SuppTable2.RFX2_rnaseq_chip.2012oct.xls : Direct target of RFX2 identified in this study (genes differentially expressed under Rfx2 knock-down + Rfx2 ChIP-seq peaks).
• DAVID6.7_RFX2_DirectDEdown_HGNC.xls : Functional modules enriched in genes down-regulated under Rfx2 knockdown + Rfx2 ChIP-seq peaks. (using DAVID 6.7)
• DAVID6.7_RFX2_DirectDEup_HGNC.xls : Functional modules enriched in genes up-regulated under Rfx2 knockdown + Rfx2 ChIP-seq peaks. (using DAVID 6.7)

Videos

• Video S1. Dynamics of GFP-IFT20 in a control multiciliated cell.
• Video S2. Dynamics of GFP-IFT20 in a Ttc29-knockdown multiciliated cell.
• Video S3. Cilia beating of a control multiciliated cell.
• Video S4. Cilia beating of a Ribc2-knockdown multiciliated cell.
• Video S5. The development of a control multiciliated cell.
• Video S6. The insertion of a control multiciliated cell into the overlying
• Video S7. The insertion of a Rfx2-knockdown multiciliated cell into the